Each specimen collected at implant surgical stage was immediately fixed in 10% neutral buffered formalin, then, processed for two staining techniques; Haematoxylin & Eosin (H&E)52 (link) and Masson–Goldner Trichrome53 (link) stains for general examination of newly formed bone and detection of unmineralized bone respectively.
Slides were examined using Olympus BX41 Phase Contrast & Darkfield Microscope (Olympus Corporation, Japan), then, sections were captured using Olympus DP20 digital microscope camera (Olympus Corporation, Japan) for examination of histological slides, which was undertaken following a fully blinded approach within each study group.
Slides were examined using Olympus BX41 Phase Contrast & Darkfield Microscope (Olympus Corporation, Japan), then, sections were captured using Olympus DP20 digital microscope camera (Olympus Corporation, Japan) for examination of histological slides, which was undertaken following a fully blinded approach within each study group.
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