In Vivo Functionality of RevCAR T-Cells

All animal procedures have been approved by the local ethics committee for animal experiments (Landesdirektion Dresden, 24–9165.40-4/2013, 24.9168.21–4/2004-1) and were performed at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) in accordance with the German regulations of animal welfare. Five-week-old male NMRI-nude immunodeficient mice (Rj:NMRI-Foxn1nu/nu) (Janvier Labs, Le Genest-Saint-Isle, France) were randomly (unblinded) assigned to experimental groups of five animals and housed in a pathogen free facility with 12 h light/dark cycle. The health status of the mice was supervised daily by husbandry personnel. To assess in vivo functionality of RevCAR T-cells, 1 × 10⁶ MOLM-13-Luc cells were injected subcutaneously alone or mixed with RevCAR T-cells (1 × 10⁶) and RevTM (17 µg) in a total volume of 100 µL PBS (Thermofisher Scientific). The optical imaging was performed with the In-Vivo Xtreme (Bruker, Nehren, Germany) as described before [15 (link),25 (link)].