The same acquisition protocol was used
in a previous study.37 (link) Briefly, 1 week
before the first PET scan, a
brain T1-weighted magnetic resonance imaging
(MRI) scan of the animal was made (Signa Excite HDTx 3.0T, GE Healthcare).38 (link) Brain PET scans were acquired using a high-resolution
animal PET scanner (SHR-38000, Hamamatsu Photonics). All nonhuman
primates underwent dynamic PET scans with arterial blood sampling.
Monkeys were anesthetized (2.5% sevoflurane) during the arterial cannulation
and their transport, but they were awake during the scans and the
head was immobilized using a fixation device. The animals were positioned
in the camera in a sitting position with stereotactic coordinates
aligned paralleled to the orbitomeatal plane.
Prior to tracer
injection, a rotating 68Ge/68Ga rod source was
used to perform the transmission scan (60 min), and its information
was used for attenuation correction of the PET images. Next, animals
were injected with (R)-[11C]verapamil
(more information inTable 2 ) at the start of the emission scan (91 min) via the saphenous vein over a period of 30 s as a single bolus.
PET images were reconstructed using the filtered back projection
method with a Hanning filter of 4.5 mm in SHR-38000 Reconstruction
software (Hamamatsu Photonics) and were composed of 49 frames (6 ×
10, 6 × 30, 12 × 60, and 25 × 180 s).
in a previous study.37 (link) Briefly, 1 week
before the first PET scan, a
brain T1-weighted magnetic resonance imaging
(MRI) scan of the animal was made (Signa Excite HDTx 3.0T, GE Healthcare).38 (link) Brain PET scans were acquired using a high-resolution
animal PET scanner (SHR-38000, Hamamatsu Photonics). All nonhuman
primates underwent dynamic PET scans with arterial blood sampling.
Monkeys were anesthetized (2.5% sevoflurane) during the arterial cannulation
and their transport, but they were awake during the scans and the
head was immobilized using a fixation device. The animals were positioned
in the camera in a sitting position with stereotactic coordinates
aligned paralleled to the orbitomeatal plane.
Prior to tracer
injection, a rotating 68Ge/68Ga rod source was
used to perform the transmission scan (60 min), and its information
was used for attenuation correction of the PET images. Next, animals
were injected with (R)-[11C]verapamil
(more information in
PET images were reconstructed using the filtered back projection
method with a Hanning filter of 4.5 mm in SHR-38000 Reconstruction
software (Hamamatsu Photonics) and were composed of 49 frames (6 ×
10, 6 × 30, 12 × 60, and 25 × 180 s).
