Three iPSC lines, reprogrammed from peripheral blood mononuclear cells (PBMCs) or dermal fibroblasts, were obtained from adults with no history of retinal disease after informed consent and approval of the study by the local ethics committee (Reference No. 11-101-0228). Two clones per iPSC line were used. Fibroblasts and PBMCs were reprogrammed according to Ref. [27 (link)] and Ref. [37 (link)].
iPSCs were maintained in mTeSRTM Plus medium (Stemcell Technologies, Cologne, Germany) with 25 µg/mL Gentamycin (Merck KGaA, Darmstadt, Germany) on plates coated with hESC-qualified Matrigel® (Corning, New York, NY, USA), and cryopreserved in CryoStor® CS10 medium (Stemcell Technologies).
iPSCs were maintained in mTeSRTM Plus medium (Stemcell Technologies, Cologne, Germany) with 25 µg/mL Gentamycin (Merck KGaA, Darmstadt, Germany) on plates coated with hESC-qualified Matrigel® (Corning, New York, NY, USA), and cryopreserved in CryoStor® CS10 medium (Stemcell Technologies).
Free full text: Click here
