Immunodetection of Eukaryotic Proteins

As primary antibodies we used: mouse monoclonal α-histidine (α-His; Roche), mouse monoclonal α-BtUb (α-Ub; SantaCruz), rabbit α-EhADH (α-EhADH18) (Díaz-Hernández et al., 2021 (link)), mouse α-EhVps32 (α-EhVps32) (Avalos-Padilla et al., 2015 (link)), mouse α-LBPA (α-LBPA; Echelon Bioscience) and mouse monoclonal α-human actin (α-actin) (kindly given by Dr. Manuel Hernández, Cell Biology Department, CINVESTAV IPN). Secondary antibodies were: HRP-labelled α-rabbit, mouse and rat IgGs (Zymed) for western blot. FITC-labelled α-rabbit and α-mouse IgGs and TRITC-labelled α-rat IgGs (Life Technologies) for immunofluorescence. For immunoelectron microscopy experiments, we used α-rat IgG, conjugated with 10 nm gold particles and α-mouse IgG, conjugated with 20 nm gold particles (TED Pella Inc).

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Galindo A., Javier-Reyna R., García-Rivera G., Bañuelos C., Montaño S., Ortega-Lopez J., Chávez-Munguía B., Salazar-Villatoro L, & Orozco E. (2021). EhVps23: A Component of ESCRT-I That Participates in Vesicular Trafficking and Phagocytosis of Entamoeba histolytica. Frontiers in Cellular and Infection Microbiology, 11, 770759.

Publication 2021

α-rat IgG

Actin Antibodies Fitc Gold Histidine Human Immunoelectron microscopy Immunofluorescence Mouse Rabbit Tritc Western blot

Corresponding Organization : Instituto Politécnico Nacional

Other organizations : Autonomous University of Sinaloa

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Variable analysis

independent variables

Mouse monoclonal α-histidine (α-His; Roche)
Mouse monoclonal α-BtUb (α-Ub; SantaCruz)
Rabbit α-EhADH (α-EhADH18) (Díaz-Hernández et al., 2021)
Mouse α-EhVps32 (α-EhVps32) (Avalos-Padilla et al., 2015)
Mouse α-LBPA (α-LBPA; Echelon Bioscience)
Mouse monoclonal α-human actin (α-actin) (kindly given by Dr. Manuel Hernández, Cell Biology Department, CINVESTAV IPN)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

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