Modulating IL-17A in Allergic Asthma

IL-17A mab (Bio X Cell: 50 μg/mouse and 100 μg/mouse) or mouse IgG isotype control antibody (50 μg/mouse and 100 μg/mouse) was administered separately via the intraperitoneal route.13 (link)19 (link) Recombinant mouse IL-17A (rmIL-17A; PeproTech) was dissolved in sterile phosphate buffered saline (PBS) and instilled at a dose of 1 μg/mouse per time.13 (link) During allergen sensitization, IL-17A mab and rmIL-17A were given every 3 days beginning from the first to the second immunization for 3 times after each sensitization in total (marked as “TDI + IL-17A mab/S” and “TDI + recombinant IL [rIL]-17A/S”), while during the TDI challenge phase, IL-17A mab and rmIL-17A were given immediately after each inhalation (marked as “TDI + IL-17A mab/C” and “TDI + rIL-17A/C”).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Chen S., Yu L., Deng Y., Liu Y., Wang L., Li D., Yang K., Liu S., Tao A, & Chen R. (2022). Early IL-17A Prevention Rather Than Late IL-17A Neutralization Attenuates Toluene Diisocyanate-Induced Mixed Granulocytic Asthma. Allergy, Asthma & Immunology Research, 14(5), 528-548.

Publication 2022

IL-17A mab rmIL-17A rIL]-17A

Allergen Cell Igg antibody Il 17a Inhalation Isotype Mouse Phosphate Saline Second immunization Sterile

Corresponding Organization :

Other organizations : Jinan University, Southern University of Science and Technology, First Affiliated Hospital of Jinan University, State Key Laboratory of Respiratory Disease, Second Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University

Top 5 similar protocols

Variable analysis

independent variables

IL-17A mab (Bio X Cell: 50 μg/mouse and 100 μg/mouse)
Mouse IgG isotype control antibody (50 μg/mouse and 100 μg/mouse)
Recombinant mouse IL-17A (rmIL-17A; PeproTech) at a dose of 1 μg/mouse per time

dependent variables

Not explicitly mentioned

control variables

Administration via the intraperitoneal route
Recombinant mouse IL-17A (rmIL-17A; PeproTech) dissolved in sterile phosphate buffered saline (PBS)

controls

Mouse IgG isotype control antibody (50 μg/mouse and 100 μg/mouse)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!