PBS washed initial inoculum of C. glabrata cells were stained with 200 µg/ml of FITC (Millipore Sigma) in carbonate buffer (0.1 M Na2CO3, 0.15 M NaCl, pH = 9.3) and incubated at 37 °C, followed by 3 times PBS washing. The THP1 macrophages were infected with MOI of 10/1 and after 3-, 6-, 24-, and 48-h pst with micafungin (0.125 µg/ml), the C. glabrata cells were released from macrophages and counterstained with 50 µg/ml AF-647-ConA (Millipore Sigma) in PBS buffer+2% BSA. The pellets were washed 3 times with PBS and subjected to flow cytometry (BD Biosciences). Double-stained yeasts were mother cells, while yeast cells only stained with AF-647 ConA represented replicated cells.
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