Quantify Candida glabrata Replication
Variable analysis
- Micafungin concentration (0.125 µg/ml)
- Percentage of double-stained (mother) C. glabrata cells
- Percentage of C. glabrata cells stained only with AF-647 ConA (replicated cells)
- Initial inoculum of C. glabrata cells
- FITC staining of C. glabrata cells
- Carbonate buffer composition (0.1 M Na2CO3, 0.15 M NaCl, pH = 9.3)
- Incubation temperature (37 °C)
- Number of PBS washes (3 times)
- MOI of C. glabrata infection of THP1 macrophages (10/1)
- Concentration of AF-647-ConA staining (50 µg/ml)
- PBS buffer + 2% BSA for staining
- Number of PBS washes after staining (3 times)
- Flow cytometry analysis (BD Biosciences)
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