Fungal Strain Transformation and Cultivation

All fungal strains used or constructed in this study are listed in Table 1. These strains were cultivated on PDA medium (pH 5.6, purchased from BD company) at 25 ℃ for 7 days and used for multiplication of the inoculum. The transformants were selected on PDAS (PDA with 0.8 M D-sorbitol, pH 5.6) supplemented with appropriate antibiotics as needed, such as 100 mg/L of hygromycin B or geneticin, and cultured for 5–7 days at 30 ℃.Table 1

Strains used in this study

Strains	Characteristics	Reference
MEFC09	Wild type, Coleophoma empetri	CGMCC 21058
MEFC09-∆ku80	MEFC09 derivate, ∆ku80::neo	[34 (link)]
MEFC09-F	MEFC09 derivate, PgpdAt-mcfF-TtrpC-hph	This study
MEFC09-H	MEFC09 derivate, PgpdAt-mcfH-TtrpC-hph	This study
MEFC09-HF	MEFC09-H derivate, PgpdAt-mcfF-TtrpC-neo	This study
MEFC09-P	MEFC09 derivate, PgpdAt-mcfP-TtrpC-hph	This study
MEFC09-S	MEFC09 derivate, PgpdAt-mcfS-TtrpC-hph	This study
MEFC09-∆CEfks1	MEFC09-∆ku80 derivate, ∆CEfks1::hph	This study
MEFC09-∆CEfks2	MEFC09-∆ku80 derivate, ∆CEfks2::hph	This study
MEFC09::CEfks2	MEFC09 derivate, PgpdAt-CEfks2-Tpgk-hph	This study
MEFC09-∆mcfJ	MEFC09-∆ku80 derivate, ∆mcfJ::hph	This study
MEFC09-J	MEFC09 derivate, PgpdAt-mcfJ-Tpgk-hph	This study
MEFC09-JFH	MEFC09-J derivate, PgpdAt-mcfF-TtrpC-neo, PgpdAt-mcfH-TtrpC-neo	This study

CGMCC: China General Microbiological Culture Collection Center

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Men P., Zhou Y., Xie L., Zhang X., Zhang W., Huang X, & Lu X. (2023). Improving the production of the micafungin precursor FR901379 in an industrial production strain. Microbial Cell Factories, 22, 44.

Publication 2023

PDA medium

Antibiotics Geneticin Hygromycin b Mcfs Sorbitol Strains

Corresponding Organization : Qingdao National Laboratory for Marine Science and Technology

Other organizations : University of Jinan, State Key Laboratory of Food Science and Technology, Nanchang University

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Variable analysis

independent variables

CEfks1
CEfks2

dependent variables

Not explicitly mentioned

control variables

Cultivation conditions: PDA medium (pH 5.6), 25 °C, 7 days
Selection conditions: PDAS (PDA with 0.8 M D-sorbitol, pH 5.6) supplemented with antibiotics (100 mg/L hygromycin B or geneticin), 30 °C, 5-7 days

positive controls

Not specified

negative controls

Not specified

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