Muscle Fiber Cross-sectional Analysis

After MAS scoring and muscle tone measurement, the rats were anesthetized by spontaneous sevoflurane inhalation and sacrificed by decapitation. The gastrocnemius muscle was dissected and weighed. Subsequently, the upper, middle, and lower parts of the INDR were stained using HE. Case Viewer software was used to measure the cross-sectional area of 500 muscle fibers within 5 fields under a microscope (the upper, lower, left, right and middle parts of the tissue section).

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Yu J., Li Y., Yang L., Li Y., Zhang S, & Yang S. (2023). The highest region of muscle spindle abundance should be the optimal target of botulinum toxin A injection to block muscle spasms in rats. Frontiers in Neurology, 14, 1061849.

Publication 2023

Decapitation Gastrocnemius muscle Inhalation Microscope Muscle Muscle tone Rats Sevoflurane Tissue

Corresponding Organization : Zunyi Medical University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Cross-sectional area of 500 muscle fibers
Gastrocnemius muscle weight

control variables

None explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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