Tick-Borne Pathogen Transmission

Ixodes ricinus larvae, nymphs and adults were obtained from the breeding facility of the Institute of Parasitology, Biology Centre, Czech Academy of Sciences. To prepare infected I. ricinus nymphs, the larvae were fed on B. afzelii CB43 infected mice and allowed to moult to nymphs. Nymphs were considered to be infected if greater than 90% of them were PCR positive (for details, see [24 (link)]). Inbred, pathogen-free C3H/HeN mice (Jackson Laboratory, Germany) were used for the pathogen transmission experiment.

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Perner J., Kucera M., Frantova H., Urbanova V., Kopacek P, & Sima R. (2022). Lyme disease transmission by severely impaired ticks. Open Biology, 12(2), 210244.

Publication 2022

C3H/HeN mice

Adults C3h mice Ixodes ricinus Larvae Mice Moult Nymphs Pathogen Pathogen transmission Ricinus

Corresponding Organization : Czech Academy of Sciences, Biology Centre

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Variable analysis

independent variables

Infection of Ixodes ricinus larvae with Borrelia afzelii CB43

dependent variables

Infection rate of Ixodes ricinus nymphs, measured by PCR positivity

control variables

Inbred, pathogen-free C3H/HeN mice (Jackson Laboratory, Germany) used for the pathogen transmission experiment

controls

Positive control: Not specified
Negative control: Not specified

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