For determination of PD-L1 protein expression, positivity was defined as complete circumferential or partial cell membrane staining of viable tumor cells with 1+ to 3+ intensity. Nonspecific staining was recorded on a 0 to 3 intensity scale, in 0.25 grade increments. Tumor-associated immune cells were excluded from PD-L1 scoring.16 Cytoplasmic staining, if present, was excluded from the scoring. Scoring was recorded as percentage of PD-L1-positive tumor cells over total tumor cells in the denominator (TPS). NSCLC specimens stained with the negative control reagent must have 0 specific membrane staining and ≤1+ intensity nonspecific (nonmembrane) staining.
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PD-L1 Protein Expression Assessment
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Corresponding Organization : Agilent Technologies (United States)
Other organizations : Merck & Co., Inc., Rahway, NJ, USA (United States)
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Variable analysis
independent variables
- PD-L1 protein expression
- Percentage of PD-L1-positive tumor cells over total tumor cells (TPS)
- Tumor-associated immune cells were excluded from PD-L1 scoring
- Cytoplasmic staining was excluded from the scoring
- NSCLC specimens stained with the negative control reagent must have 0 specific membrane staining and ≤1+ intensity nonspecific (nonmembrane) staining
- Negative control reagent
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