Immunofluorescence Staining of iTreg Cells

Cell suspension from naïve and iTreg skewing cells stained as described ^{45 (link)} using mouse anti-CD4 (BD-Pharmigen), rabbit anti-Smyd3 (Abcam), goat anti-rabbit Alexa Fluor 568, and anti-mouse Alexa Fluor 488 (Invitrogen). The stained tissue sections were analyzed using a Nikon A1 confocal microscope system (Nikon Instruments).

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de Almeida Nagata D.E., Ting H.A., Cavassani K.A., Schaller M.A., Mukherjee S., Ptaschinski C., Kunkel S.L, & Lukacs N.W. (2015). Epigenetic control of Foxp3 by SMYD3 H3K4 histone methyltransferase controls iTreg development and regulates pathogenic T cell responses during pulmonary viral infection. Mucosal immunology, 8(5), 1131-1143.

Publication 2015

mouse anti-CD4 rabbit anti-Smyd3 goat anti-rabbit Alexa Fluor 568 anti-mouse Alexa Fluor 488 Nikon A1 confocal microscope system

Alexa 568 Alexa fluor 488 Cells Confocal microscope Goat Mouse Rabbit Stained tissue

Corresponding Organization :

Other organizations : University of Michigan–Ann Arbor

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Variable analysis

independent variables

Naïve cell skewing
ITreg cell skewing

dependent variables

Expression of CD4
Expression of Smyd3

control variables

Staining protocol
Imaging system

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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