Histological Examination of Colon Tissue

Five-mm segments of proximal and distal colon were resected and fixed in 10% neutral buffered formalin for histological examination as described previously (57 (link)). Briefly, tissues were embedded in paraffin wax, sectioned into 4-μm-thick sections with a paraffin microtome (ThermoFisher), and mounted on microscope slides (Fisher Scientific). Dewaxed sections were stained with H&E (Richard Alen Scientific). Histological images were obtained using a Zeiss Axio Observer microscope with an Olympus DP72 camera (Zeiss).

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Rais R., Jiang W., Zhai H., Wozniak K.M., Stathis M., Hollinger K.R., Thomas A.G., Rojas C., Vornov J.J., Marohn M., Li X, & Slusher B.S. (2016). FOLH1/GCPII is elevated in IBD patients, and its inhibition ameliorates murine IBD abnormalities. JCI Insight, 1(12), e88634.

Publication 2016

paraffin microtome microscope slides Axio Observer microscope

Colon Formalin Microscope Microtome Paraffin Tissues

Corresponding Organization : Johns Hopkins University

Other organizations : Union Hospital, Huazhong University of Science and Technology, Medpace (United States)

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Variable analysis

independent variables

Proximal colon segment
Distal colon segment

dependent variables

Histological characteristics

control variables

Tissue fixation in 10% neutral buffered formalin
Paraffin embedding
Tissue sectioning at 4-μm thickness
H&E staining

controls

Positive control: Not specified
Negative control: Not specified

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