Tryptic peptides were subject to LC-MS/MS analysis using an Agilent 1100 LC system (Santa Clara, CA) connected to a Finnigan LTQ ion trap mass spectrometer (Thermo Fisher Scientific, Inc., San Jose, CA), as described previously 20 (link). Briefly, the peptide mixture was injected, using an autosampler (Agilent), and loaded onto a C18 peptide trap (Agilent). After washing, peptides were eluted from the trap with a gradient of acetonitrile (0 – 60% in 35 min) at a flow rate of 250 nL/min. The eluted peptides were then separated in a C18 PicoFrit column (New Objectives, Boston, MA) positioned directly in front of the orifice of an ion transfer tube of the LTQ mass spectrometer. Spectra were acquired in a data-dependent manner with dynamic exclusion option enabled. Each survey MS scan was followed by five MS/MS scans.
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Tryptic Peptide Analysis by LC-MS/MS
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Other organizations : Virginia Commonwealth University, National Institute on Aging, National Institutes of Health, Virginia Commonwealth University Medical Center, National Heart Lung and Blood Institute
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- LC-MS/MS analysis using an Agilent 1100 LC system connected to a Finnigan LTQ ion trap mass spectrometer
- Tryptic peptides
- Gradient of acetonitrile (0 – 60% in 35 min) at a flow rate of 250 nL/min
- C18 peptide trap (Agilent)
- C18 PicoFrit column (New Objectives, Boston, MA)
- Dynamic exclusion option enabled
- Each survey MS scan followed by five MS/MS scans
- No positive or negative controls were explicitly mentioned in the provided information.
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