To purify cardiomyocytes, a variant of RPMI 1640 without D-glucose (11879, Life Technologies) was used in the medium formula. RPMI without D-glucose was supplemented with 213 µg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich), 500 µg/mL Oryza sativa-derived recombinant human albumin (Sigma-Aldrich), and 5 mM sodium DL-lactate (L4263, Sigma-Aldrich) and used in place of CDM3 on differentiation days 10–20.
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Cardiomyocyte Purification Protocol
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Corresponding Organization : Stanford University
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Variable analysis
independent variables
- Use of RPMI 1640 without D-glucose in the medium formula
- Purification of cardiomyocytes
- 213 µg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich)
- 500 µg/mL Oryza sativa-derived recombinant human albumin (Sigma-Aldrich)
- 5 mM sodium DL-lactate (L4263, Sigma-Aldrich)
- No positive or negative controls were explicitly mentioned.
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