Flow cytometry was employed to verify the varying levels of CD38 expression by the lung cancer cell lines. Daratumumab served as the primary antibody, at a concentration of 20 μg of antibody per 1 mL of solution, while goat anti-human AlexaFluor488 secondary antibody was utilized. Proper controls of cells alone, primary antibody alone, secondary antibody alone, and a nonspecific IgG antibody were employed. Staining and flow cytometry analysis followed standard protocols.24 (link) Analysis was performed using the MACSQuant cytometer and FlowJo (V.10) software.
Protocol detail
Quantifying CD38 Expression in Lung Cancer
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Anti antibody
Antibody
Cell lines
Cells
Daratumumab
Flow cytometry
Goat
Human
Igg antibody
Lung cancer
Corresponding Organization :
Other organizations : University of Wisconsin–Madison, University of Wisconsin Carbone Cancer Center
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Variable analysis
independent variables
- Concentration of daratumumab primary antibody (20 μg/mL)
- Levels of CD38 expression by the lung cancer cell lines
- Cells alone
- Primary antibody alone
- Secondary antibody alone
- Nonspecific IgG antibody
- Positive controls: Cells alone, primary antibody alone, secondary antibody alone
- Negative control: Nonspecific IgG antibody
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