Knockdown of WWP2 and WWP1 in Cells

Vectors containing a control short hairpin RNA (shRNA) and WWP2 shRNA (shRNA1, 5′-CAGGAUGGGAGAUGAAAUAUU-3′; shRNA2, 5′-ACAUGGAGAUACUGGGCAAUU-3′), WWP1 shRNA (shRNA1, 5′-ATTGCTTATGAACGCGGCT-3′; shRNA 2, ACAACACACCTTCATCTCC-3′) were purchased from Open Biosystems and were transfected into cells with Lipofectamine using standard protocols. WWP2 siRNA described earlier (23 (link)) and prevalidated siRNAs for PPM1G (catalog numbers S102658684 and S102658691) were purchased from Qiagen and transfected using Oligofectamine using standard protocols.

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Chaudhary N, & Maddika S. (2014). WWP2-WWP1 Ubiquitin Ligase Complex Coordinated by PPM1G Maintains the Balance between Cellular p73 and ΔNp73 Levels. Molecular and Cellular Biology, 34(19), 3754-3764.

Publication 2014

shRNA shRNA1 shRNA2

Cells Lipofectamine Oligofectamine Shrna Sirnas Vectors

Corresponding Organization :

Other organizations : Centre for DNA Fingerprinting and Diagnostics

Top 5 similar protocols

Variable analysis

independent variables

Control short hairpin RNA (shRNA)
WWP2 shRNA (shRNA1 and shRNA2)
WWP1 shRNA (shRNA1 and shRNA2)
WWP2 siRNA
PPM1G siRNA

dependent variables

Measured outcomes (not explicitly mentioned)

control variables

Transfection protocols using Lipofectamine and Oligofectamine

controls

Positive control: None specified
Negative control: Control short hairpin RNA (shRNA)

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