Double-immunofluorescence staining of cerebral cortex was performed at 24 h after SAH as described previously (Xie et al., 2017 (link)). Sections were incubated overnight at 4°C with rabbit anti-ErbB4 (Abcam, Cambridge, MA, United States), rabbit anti-YAP (Cell Signaling Technology, Beverly, MA, United States), and mouse anti-CD31 (Abcam, Cambridge, MA, United States), followed by fluorescence dye-conjugated secondary antibodies (Jackson Immunoresearch, West Grove, PA, United States) for 3 h at 20°C. The sections were then visualized with a fluorescence microscope. Photomicrographs were analyzed using Image-Pro Plus software (Olympus, Melville, NY, United States).
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