Plumericin was isolated from bark material of Himatanthus sucuuba, as described earlier66 (link). PHA-408 was obtained from Axon Medchem BV (Groningen, Netherlands) and NAC, DPI, Triton X-100, medium M199, fetal bovine serum (FBS), goat serum, gelatin, and paraformaldehyde (PFA) were purchased from Sigma Aldrich (Saint Louis, USA). Penicillin, streptomycin, fungizone, and trypsin-EDTA were bought from LONZA (Visp, Switzerland), endothelial cell growth supplement (ECGS) with heparin from PromoCell (Heidelberg, Germany), and human recombinant TNFα from PeproTech (Vienna, Austria). The products 2′,7′-dichlorodihydrofluresceindiacetate (H2-DCF) and TMB ELISA substrate were obtained from ThermoFischer Scientific (Vienna, Austria). Hoechst 33342 and Alexa 555 were obtained from Life Technologies (Columbus, USA), mouse IgG HRP-linked antibody from GE Health care (Little Chalfont, UK), anti-CD31 and anti-p16 from BioLegend (San Diego, USA), anti-p65 from Santa Cruz (Santa Cruz, USA), anti-Ki-67 from ThermoFischer Scientific, anti-p21 from BD Bioscience (Franklin Lakes, USA), anti-E selectin and anti-ICAM-1 from R&D Systems (Abingdon, UK). Trizol, MuLV-reverse transcriptase, RNAse inhibitor and, oligo dT primers were obtained from Life Technologies, and FastStart SYBR Green Master Mix from ThermoFischer Scientific.
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