HCV Transgenic Mice for Inducible Cre Expression

R6CN2HCV transgenic mice that possess HCVR6 (genotype 1b) cDNA (nucleotides 294 to 3435) downstream of the CAG promoter, the neomycin resistance gene (neo), and poly(A) signal flanked by two loxP sequences (12 (link)) were bred with Mx1-Cre transgenic mice (purchased from The Jackson Laboratory) to produce R6CN2HCV-MxCre transgenic mice (CN2-29^+/−/MxCre^+/−), here referred to as HCV transgenic mice. Cre expression in the livers of these mice was induced by intraperitoneal injection of poly(I · C) (GE Healthcare UK Ltd., Buckinghamshire, England); 300 μl poly(I · C) solution {1 mg/ml calcium- and magnesium-free phosphate-buffered saline [in PBS(−)]} was injected 3 times at 48-h intervals.

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Ohtsuki T., Kimura K., Tokunaga Y., Tsukiyama-Kohara K., Tateno C., Hayashi Y., Hishima T, & Kohara M. (2015). M2 Macrophages Play Critical Roles in Progression of Inflammatory Liver Disease in Hepatitis C Virus Transgenic Mice. Journal of Virology, 90(1), 300-307.

Publication 2015

Mx1-Cre transgenic mice poly(I · C)

Calcium Cdna Gene Genotype Intraperitoneal injection Livers Magnesium phosphate Mice Neomycin Nucleotides Poly Saline Transgenic mice

Corresponding Organization :

Other organizations : Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Komagome Hospital, Kagoshima University, PhoenixBio (Japan)

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Variable analysis

independent variables

Cre expression in the livers of HCV transgenic mice (CN2-29+/−/MxCre+/−) induced by intraperitoneal injection of poly(I · C)

dependent variables

Not explicitly mentioned

control variables

Genotype of HCV transgenic mice (CN2-29+/−/MxCre+/−)
Dose and frequency of poly(I · C) injection (300 μl poly(I · C) solution {1 mg/ml calcium- and magnesium-free phosphate-buffered saline [in PBS(−)]} injected 3 times at 48-h intervals)

controls

Positive control: Mx1-Cre transgenic mice purchased from The Jackson Laboratory
Negative control: Not explicitly mentioned

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