Treg Cell Depletion in Mice

In vivo depletion of Treg cells with anti-CD25 antibodies was performed as previously described [9 (link)]. We verified that this schedule was very effective in the depletion of Treg cells without causing significant alterations in other T cell subsets. Briefly, C57BL/6 Foxp3^GFP mice were given i.p. injections of 500 μg of anti-CD25 (clone PC61) or control rat IgG (BioXcell, USA) diluted in sterile PBS. Antibodies were administered on days -3 and +3 relative to infection with P. brasiliensis yeasts.

Free full text: Click here

Bazan S.B., Costa T.A., de Araújo E.F., Feriotti C., Loures F.V., Pretel F.D, & Calich V.L. (2015). Loss- and Gain-of-Function Approaches Indicate a Dual Role Exerted by Regulatory T Cells in Pulmonary Paracoccidioidomycosis. PLoS Neglected Tropical Diseases, 9(10), e0004189.

Publication 2015

anti-CD25 (clone PC61) control rat IgG

Anti antibodies Antibodies C57bl mice Cd25 Clone Infection Sterile T cell subsets Treg cells Yeasts

Corresponding Organization :

Other organizations : Universidade de São Paulo

Top 5 similar protocols

Variable analysis

independent variables

Administration of anti-CD25 antibody (clone PC61) or control rat IgG

dependent variables

Depletion of Treg cells
Alterations in other T cell subsets

control variables

Mouse strain: C57BL/6 Foxp3^GFP
Route of administration: i.p. injections
Dose of antibodies: 500 μg
Timing of antibody administration: days -3 and +3 relative to infection with P. brasiliensis yeasts

controls

Positive control: Anti-CD25 antibody (clone PC61)
Negative control: Control rat IgG

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!