10 ml of 10 % aqueous gelatin solution was mixed rapidly with 0.5 ml of 25 % glutaraldehyde solution at room temperature, and a filter paper disk (diameter: 10 cm) was soaked with the mixture. Then the disk was placed between two glass plates covered with polyethylene foils. Spacers were applied to produce a 0.5 mm thick membrane. After a 2 hour setting time the filter paper reinforced gelatin membrane was removed from the form and it was placed into distilled water overnight before the measurements.
Mechanically Reinforced Gelatin Membrane
10 ml of 10 % aqueous gelatin solution was mixed rapidly with 0.5 ml of 25 % glutaraldehyde solution at room temperature, and a filter paper disk (diameter: 10 cm) was soaked with the mixture. Then the disk was placed between two glass plates covered with polyethylene foils. Spacers were applied to produce a 0.5 mm thick membrane. After a 2 hour setting time the filter paper reinforced gelatin membrane was removed from the form and it was placed into distilled water overnight before the measurements.
Corresponding Organization :
Other organizations : Budapest University of Technology and Economics, Jósa András Múzeum, Szent Imre Egyetemi Oktatókórház, Semmelweis University
Protocol cited in 6 other protocols
Variable analysis
- Concentration of gelatin solution
- Concentration of glutaraldehyde solution
- Thickness of the membrane
- Mechanical strength of the membrane
- Cellulose in the filter paper (as it does not react with ClO2)
- Room temperature
- Soaking time of the filter paper in the gelatin-glutaraldehyde mixture
- Overnight storage of the membrane in distilled water before measurements
- Positive control: Not mentioned
- Negative control: Not mentioned
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!