Peptide Synthesis of FliC and FliD

NCP Block of 96 hydroxypropylmethacrylate pins and F-moc protected amino acids were obtained from Mimotopes (Clayton, Victoria, Australia). Chemicals used for synthesis and side chain deprotection like piperidine, dimethylformamide (DMF), trifluoracetic acid, diisopropylcarbodiimide (DIC) were of analytical grade, purchased from Merck Millipore (Burlington, MA, USA). Reagents like 2-mercaptoethanol, ethanedithiol, anisole, 1-hydroxybenzotriazole (HoAt), N,N-diisopropylethylamine (DIEA) and bromophenol blue were purchased from Sigma-Aldrich (Saint Louis, MO, USA).
A total of 20 FliC 15-amino acid- and 32 FliD 16-amino acid-long peptides covering predicted epitopes were synthesized. Peptides were synthesized according to standard protocol^{25 (link)} with slight changes^{6 (link),26 (link)}.