Antarctic Krill Genome Sequencing

Our experimental procedures complied with the current laws on animal welfare and research in China. Alive Antarctic krill specimens were collected from the Argentine Sea area (45°92’S,61°82’W). Genomic DNAs were extracted from the whole bodies of pooled specimens with a Qiagen GenomicTip100 kit (Qiagen, Germanton, MD, USA) according to the manufacturer’s protocol. With the traditional whole-genome shotgun sequencing strategy [14 (link)], we used 1 μg of normalized DNA to prepare a paired-end short-insert library (400 bp). Quantification and size estimations of the library were performed on a Zebra Flowcell 3.1 chip. Finally, the library was normalized to 15 ng/μL for paired-end sequencing (100 bp in length) on a BGISeq500 platform (BGI, Shenzhen, China). Raw genome sequencing reads have been deposited in the NCBI and China National GeneBank (CNGB) under the project IDs PRJNA598052 and CNP0000808, respectively.

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Huang Y., Bian C., Liu Z., Wang L., Xue C., Huang H., Yi Y., You X., Song W., Mao X., Song L, & Shi Q. (2020). The First Genome Survey of the Antarctic Krill (Euphausia superba) Provides a Valuable Genetic Resource for Polar Biomedical Research. Marine Drugs, 18(4), 185.

Publication 2020

GenomicTip100 kit

Bodies Bp 100 Chip Genome Krill Library Zebra

Corresponding Organization : Dalian Ocean University

Other organizations : Ocean University of China, Chinese Academy of Fishery Sciences

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Variable analysis

independent variables

Alive Antarctic krill specimens were collected from the Argentine Sea area (45°92'S,61°82'W).

dependent variables

Genomic DNAs were extracted from the whole bodies of pooled specimens.
Quantification and size estimations of the library were performed on a Zebra Flowcell 3.1 chip.
The library was normalized to 15 ng/μL for paired-end sequencing (100 bp in length) on a BGISeq500 platform.

control variables

Alive Antarctic krill specimens were collected.
A Qiagen GenomicTip100 kit (Qiagen, Germanton, MD, USA) was used to extract genomic DNAs according to the manufacturer's protocol.
1 μg of normalized DNA was used to prepare a paired-end short-insert library (400 bp).

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