Acti-stain Phalloidin Extracellular Matrix Imaging

Acti-stain
488 phalloidin was purchased from Cytoskeleton Inc. Advanced Dulbecco’s
modified Eagle’s medium (aDMEM), Dulbecco’s modified
Eagle’s medium–Nutrient Mixture F-12 l-glutamine
(DMEM/F12), Dulbecco’s phosphate-buffered saline (DPBS) 10×,
glutamine, horse serum, penicillin–streptomycin, phosphate-buffered
saline (PBS), propidium iodide (PI), and Vybrant DiO cell-labeling
solution were acquired from Thermo Fisher Scientific. Anti-Rabbit
IgG (H+L), CF 647 antibody produced in goat (SAB4600184), bovine serum
albumin (BSA), calcein AM, cholera toxin, doxorubicin, epidermal growth
factor (EGF), fetal bovine serum (FBS), hydrocortisone, insulin from
bovine pancreas, paraformaldehyde (PFA), Triton X-100, and 4′,6-diamidino-2-phenylindole
(DAPI) were purchased from Sigma-Aldrich. Anti-collagen I antibody
(ab34710), anti-collagen III antibody (ab7778), anti-collagen IV antibody
(ab6586), anti-fibronectin antibody (ab2413), and goat serum were
purchased from Abcam. Collagen type I (Col1) was isolated from rat
tail tendons.^{68 (link)} The Col1 content was measured
by microBCA (Thermo Fisher Scientific) as previously described.^{56 (link)}