Florets were isolated from fixed capitula, dehydrated in a graded ethanol series (70–100 %) and incubated in solutions of absolute ethanol and methyl salicylate (Sigma-Aldrich) (3:1, 1:1, 1:3, v/v) and in two changes of pure methyl salicylate according to protocol described by Musiał et al. (2015 (link)). The cleared plant material was analysed under a Nikon Eclipse 80i microscope fitted with Nomarski’s interference contrast (DIC optics).
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