Cell viability was assessed using CellTiter-Glo (Promega, Madison, Wisconsin, USA). Briefly, 1000 cells were seeded in white, flat-bottom, 96-well plates (Corning, Corning, NY, USA). After 24 h, drugs were added to the medium, and cells were incubated for 72 h. CellTiter-Glo luminescent reagent was added according to the manufacturers protocol, and the luminescence signal measured on a Synergy LX (Agilent, California, USA) with BioTek Gen5 (v3.08). To evaluate if a combination of drugs is synergistic, cells were simultaneously treated with varying concentrations of drugs, and cell viability was measured with CellTiter-Glo. Synergism scores were obtained using the R package SynergyFinder (v2.2.4)75 (link).
Dorado García H., Pusch F., Bei Y., von Stebut J., Ibáñez G., Guillan K., Imami K., Gürgen D., Rolff J., Helmsauer K., Meyer-Liesener S., Timme N., Bardinet V., Chamorro González R., MacArthur I.C., Chen C.Y., Schulz J., Wengner A.M., Furth C., Lala B., Eggert A., Seifert G., Hundsoerfer P., Kirchner M., Mertins P., Selbach M., Lissat A., Dubois F., Horst D., Schulte J.H., Spuler S., You D., Dela Cruz F., Kung A.L., Haase K., DiVirgilio M., Scheer M., Ortiz M.V, & Henssen A.G. (2022). Therapeutic targeting of ATR in alveolar rhabdomyosarcoma. Nature Communications, 13, 4297.
Other organizations :
Charité - Universitätsmedizin Berlin, Memorial Sloan Kettering Cancer Center, Max Delbrück Center, Humboldt-Universität zu Berlin, Freie Universität Berlin, Bayer (Germany)
Microplate type (white, flat-bottom, 96-well plates)
Measurement instrument (Synergy LX with BioTek Gen5 software)
positive controls
Not specified
negative controls
Not specified
Annotations
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