Head and Neck Irradiation in Mice

The head and neck region (full-body shielded) of mice from each genotype were irradiated using a Varian 6 MV linear accelerator, at a dose rate of 200 cGy/min, according to previously published methods (15 (link)). Groups of mice received a single 30 Gy fraction, and other groups received 10 Gy daily fractions for four consecutive days. Mice were immobilized and irradiated while under Nembutal anesthesia as described elsewhere (14 (link), 15 (link)). Lower single- or fractionated radiation doses did not induce mucositis (14 (link), 15 (link)).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Willis J., Epperly M.W., Fisher R., Zhang X., Shields D., Hou W., Wang H., Li S., Wipf P., Parmar K., Guinan E., Steinman J, & Greenberger J.S. (2018). Amelioration of Head and Neck Radiation-Induced Mucositis and Distant Marrow Suppression in Fanca−/− and Fancg−/− Mice by Intraoral Administration of GS-Nitroxide (JP4-039). Radiation research, 189(6), 560-578.

Publication 2018

6 MV linear accelerator

Anesthesia Body Genotype Head Linear accelerator Mice Mucositis Neck Nembutal Radiation

Corresponding Organization :

Other organizations : UPMC Hillman Cancer Center, University of Pittsburgh, Dana-Farber Cancer Institute

Top 5 similar protocols

Variable analysis

independent variables

Radiation dose (single 30 Gy fraction or 10 Gy daily fractions for four consecutive days)

dependent variables

Mucositis induction

control variables

Genotype of mice
Head and neck region (full-body shielded) of mice irradiated
Radiation dose rate (200 cGy/min)
Anesthesia (Nembutal)
Immobilization of mice during irradiation

controls

Lower single- or fractionated radiation doses (which did not induce mucositis)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!