Neurodegeneration Marker Analysis in SMA

CSF was collected in polypropylene tubes and was immediately analyzed for routine CSF parameters within 1 hour after lumbar puncture. Cell count and analysis of routine parameters was done as earlier described.^{18 (link)} For measurement of neurodegeneration markers, CSF was frozen within 1 hour after lumbar puncture and stored at −80° until analysis. Commercially available enzyme-linked immunosorbent assay (ELISA) kits were used to determine the concentrations of CSF NfL (IBL, Hamburg, Germany) and pNfH (Biovendor, Heidelberg, Germany).^{11 (link),12} A total of 50 CSF and 50 serum samples of 25 SMA patients, and 25 CSF and 25 serum samples of 25 controls were analyzed.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Wurster C.D., Günther R., Steinacker P., Dreyhaupt J., Wollinsky K., Uzelac Z., Witzel S., Kocak T., Winter B., Koch J.C., Lingor P., Petri S., Ludolph A.C., Hermann A, & Otto M. (2019). Neurochemical markers in CSF of adolescent and adult SMA patients undergoing nusinersen treatment. Therapeutic Advances in Neurological Disorders, 12, 1756286419846058.

Publication 2019

CSF NfL pNfH

Enzyme linked immunosorbent assay Frozen Lumbar puncture Neurodegeneration Patients Polypropylene Serum

Corresponding Organization :

Other organizations : Universität Ulm, German Center for Neurodegenerative Diseases, TU Dresden, Zimmer Biomet (Germany), Klinikum rechts der Isar, Medizinische Hochschule Hannover, University of Rostock

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

None specified

dependent variables

Concentrations of CSF NfL and pNfH

control variables

Time between lumbar puncture and analysis of CSF samples (within 1 hour)
Storage of CSF samples at -80°C until analysis
Use of commercially available ELISA kits to measure NfL and pNfH concentrations

controls

Positive control: 25 CSF and 25 serum samples from SMA patients
Negative control: 25 CSF and 25 serum samples from 25 controls

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!