For the transcriptomic study of A. fumigatus WT and ΔsscA conidia, each strain was streaked onto solid MMY and grown at 37 °C for 2 days. Conidia of each strain were then suspended with 0.02% Triton X-100 (Thermo Scientific, USA) and filtered with Miracloth (Merck, USA).
Total RNA extraction was performed as previously described (Son and Park 2020 (link)). All samples were isolated using a Mini-bead beater (BioSpec Products Inc., USA) and TRIzol (GeneAll Biotechnology, Republic of Korea), and the extracted RNA samples were dissolved in diethylpyrocarbonate (DEPC)-treated water (Bioneer, Republic of Korea). Total RNA was treated with RQ1 RNase-free DNase (Promega, USA) and purified using the RNeasy Mini Kit (Qiagen, Germany). RNA sequencing was performed by Theragen Bio (Suwon, Republic of Korea). Briefly, three biological replicates of each strain were sequenced on Illumina Novaseq 6000, and reads were annotated with the A. fumigatus AF293 transcriptome using the aligner STAR v.2.3.0e software. The DESeq2 method was used for evaluating and normalising differentially expressed genes (DEGs). DEGs were screened with |log2(fold change)| ≥ 1 and p value < 0.05, and gene ontology (GO) analyses were performed using the R package and FungiDB database.
Total RNA extraction was performed as previously described (Son and Park 2020 (link)). All samples were isolated using a Mini-bead beater (BioSpec Products Inc., USA) and TRIzol (GeneAll Biotechnology, Republic of Korea), and the extracted RNA samples were dissolved in diethylpyrocarbonate (DEPC)-treated water (Bioneer, Republic of Korea). Total RNA was treated with RQ1 RNase-free DNase (Promega, USA) and purified using the RNeasy Mini Kit (Qiagen, Germany). RNA sequencing was performed by Theragen Bio (Suwon, Republic of Korea). Briefly, three biological replicates of each strain were sequenced on Illumina Novaseq 6000, and reads were annotated with the A. fumigatus AF293 transcriptome using the aligner STAR v.2.3.0e software. The DESeq2 method was used for evaluating and normalising differentially expressed genes (DEGs). DEGs were screened with |log2(fold change)| ≥ 1 and p value < 0.05, and gene ontology (GO) analyses were performed using the R package and FungiDB database.
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