Quantitative Western Blot Analysis

The density of protein bands detected by Western blotting was measured using ImageJ (National Institutes of Health, Bethesda, MD, USA). For overexpression, siRNA, or cell viability assays, biological replicates were performed using three different samples. Three technical replicates were performed for the pull-down assay of HPSB5 and PLK2. Statistical significance was determined at p < 0.05 on Student’s t-test using Excel 2019 (Microsoft Japan, Tokyo, Japan). Multiple comparisons were performed using the Tukey method with BellCurve (Social Survey Research Information, Tokyo, Japan) [52 (link)].