Phytohormone Extraction and Quantification

Fresh rapeseed samples were prepared to obtain the phytohormone extract [61 (link)]. The standard auxin (indole-3-acetic acid, IAA), cytokinin (CTK), gibberellin (GA), abscisic acid (ABA), and jasmonic acid (JA), and ABA were purchased from Sigma-Aldrich (St. Louis, MO, USA) or OlChemIm (OlChemIm, Olomouc, Czech Republic). The phytohormone concentrations were determined by ultra-fast liquid chromatography-electrospray ionization tandem mass spectrometry (UFLC-ESI-MS) [62 (link)].

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Feng Y.N., Cui J.Q., Zhou T., Liu Y., Yue C.P., Huang J.Y, & Hua Y.P. (2020). Comprehensive dissection into morpho-physiologic responses, ionomic homeostasis, and transcriptomic profiling reveals the systematic resistance of allotetraploid rapeseed to salinity. BMC Plant Biology, 20, 534.

Publication 2020

gibberellin abscisic acid jasmonic acid

Abscisic acid Auxin Cytokinin Electrospray ionization mass spectrometry Gibberellin Indole 3 acetic acid Jasmonic acid Liquid chromatography Phytohormone

Corresponding Organization :

Other organizations : Zhengzhou University

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Variable analysis

independent variables

Preparation of fresh rapeseed samples

dependent variables

Phytohormone concentrations (auxin, cytokinin, gibberellin, abscisic acid, jasmonic acid)

control variables

Standard auxin (indole-3-acetic acid, IAA)
Standard cytokinin (CTK)
Standard gibberellin (GA)
Standard abscisic acid (ABA)
Standard jasmonic acid (JA)

controls

Positive controls: Standard auxin, cytokinin, gibberellin, abscisic acid, and jasmonic acid purchased from Sigma-Aldrich or OlChemIm
Negative controls: Not explicitly mentioned

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