The ATP level was measured in cell lysates as previously reported41 (link). Briefly, cells were detached with trypsin, washed once with HBSS, and resuspended in a buffer containing 0.25 M sucrose, 50 mM HEPES, 0.5 mM EDTA, 4 mM MgSO4, pH 7.4. Aliquots of cells were lysed in DMSO and total cellular ATP was measured using a Luciferin–Luciferase assay kit (ATP bioluminescent assay kit, Merck KGaA, Darmstadt, Germany) according to the manufacturer’s protocol. The amount of ATP measured was normalized to the protein content determined by a modified protocol of the Lowry method as reported42 (link).
Positive control: Luciferin–Luciferase assay kit for ATP measurement
Negative control: Not explicitly mentioned
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