Protocol detail

Microbial Profiling of Dairy Products

Find Similar Protocols

Milk samples were directly serially diluted (1:10) in Ringer’s solution (Sigma-Aldrich, Milan, Italy) into 10-mL/volume test tubes, while the curd and cheese samples (10 g) were first, homogenized in sodium citrate (2% w/v) solution by means of BagMixer^® 400 (Interscience, Saint Nom, France) at the highest speed for 2 min and then serially diluted in Ringer’s solution. The dilutions of raw milk and pasteurized milk were plated on agar media to analyze the total mesophilic microorganisms (TMM), and the main pro-technological (lactic acid bacteria (LAB), including enterococci); spoilage (Pseudomonas spp.) and pathogenic (members of the Enterobacteriaceae family, coagulase-positive staphylococci (CPS), Listeria monocytogenes, Escherichia coli and Salmonella spp.) bacterial groups following the approach of Gaglio et al. [28 (link)].

Free full text: Click here

Busetta G., Ponte M., Barbera M., Alfonzo A., Ioppolo A., Maniaci G., Guarcello R., Francesca N., Palazzolo E., Bonanno A., Moschetti G., Settanni L, & Gaglio R. (2022). Influence of Citrus Essential Oils on the Microbiological, Physicochemical and Antioxidant Properties of Primosale Cheese. Antioxidants, 11(10), 2004.

Publication 2022

Ringer’s solution BagMixer® 400

Agar Bacterial Cheese Coagulase Dilutions Enterobacteriaceae Enterococci Escherichia coli Family members Lactic acid bacteria Listeria monocytogenes Milk Pathogenic Pseudomonas Ringer solution Salmonella Sodium citrate Staphylococci

Top 5 similar protocols

Variable analysis

independent variables

Dilution method (direct serial dilution vs. homogenization in sodium citrate solution and then serial dilution)
Sample type (raw milk, pasteurized milk, curd, cheese)

dependent variables

Total mesophilic microorganisms (TMM)
Lactic acid bacteria (LAB) including enterococci
Spoilage bacteria (Pseudomonas spp.)
Pathogenic bacteria (Enterobacteriaceae, coagulase-positive staphylococci, Listeria monocytogenes, Escherichia coli, Salmonella spp.)

control variables

Ringer's solution (Sigma-Aldrich, Milan, Italy) used for dilutions
Sodium citrate (2% w/v) solution used for homogenization of curd and cheese samples
BagMixer® 400 (Interscience, Saint Nom, France) used for homogenization at highest speed for 2 minutes

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!