C. parvum Iowa II (IIa) strain oocysts were obtained from Bunch Grass Farms. A C. tyzzeri strain isolated from laboratory mice at Washington University in St. Louis was kindly provided by Dr. Chyi-Song Hsieh, University of Washington [39 (link)]. Parasites were maintained in 6–8 week old male and female Ifnγ−/− (Jackson Laboratory stock no. 002287) or C57BL/6 (Ct) mice bred in-house. 6–8 week old mice were handled, treated with antibiotics, and infected as detailed in [23 (link)].
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Cryptosporidium Infection Protocol in Mice
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Corresponding Organization : University of Pennsylvania
Other organizations : Houston Methodist, Scripps Institution of Oceanography, Broad Institute, University of Georgia
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Variable analysis
independent variables
- Cryptosporidium species (C. parvum and C. tyzzeri)
- Mouse strain (Ifnγ^-/- and C57BL/6)
- Parasite maintenance in mice
- Mouse age (6-8 weeks)
- Mouse sex (male and female)
- Antibiotics treatment of mice
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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