Protein Analysis and Epithelial-Mesenchymal Transition

Protein preparation and Western blot analysis were performed as described previously (33 (link)). The antibodies used in the study were as follows: Chemerin (Abcam, dilution of 1:500), GAPDH, E-cadherin, N-cadherin, Vimentin, p-JAK2, JAK2, Phospho-Rb (Abcam, dilution of 1:1000), p-STAT3, STAT3, Phospho-Rb (Abcam, dilution of 1:2000), E2F1, Cyclin D1, Cyclin E1 (Abcam, dilution of 1:1000), Slug and Snail (ProteinTech, dilution of 1:1000), and β-actin (ProteinTech, dilution of 1:4000).

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Hu X., Xiang F., Feng Y., Gao F., Ge S., Wang C., Zhang X, & Wang N. (2022). Neutrophils Promote Tumor Progression in Oral Squamous Cell Carcinoma by Regulating EMT and JAK2/STAT3 Signaling Through Chemerin. Frontiers in Oncology, 12, 812044.

Publication 2022

Chemerin GAPDH E-cadherin N-cadherin Vimentin p-JAK2 Phospho-Rb p-STAT3 STAT3 Cyclin D1 Cyclin E1 β-actin

Actin Antibodies Cyclin d1 Cyclin e1 Dilution E cadherin E2f1 Gapdh Jak2 N cadherin Protein Slug Snail Stat3 Vimentin Western blot analysis

Corresponding Organization : Qingdao University

Other organizations : Jiangxi Pingxiang People's Hospital, Affiliated Hospital of Qingdao University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Chemerin protein expression
GAPDH protein expression
E-cadherin protein expression
N-cadherin protein expression
Vimentin protein expression
P-JAK2 protein expression
JAK2 protein expression
Phospho-Rb protein expression
P-STAT3 protein expression
STAT3 protein expression
E2F1 protein expression
Cyclin D1 protein expression
Cyclin E1 protein expression
Slug protein expression
Snail protein expression
β-actin protein expression

control variables

None explicitly mentioned

controls

Positive controls: None specified
Negative controls: None specified

Annotations

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