Optogenetic activation or inhibition experiments were performed 3 weeks after viral expression. Chronically implantable fibers were connected to a laser generator using optic fiber sleeves. A Master-8 pulse stimulator (Shanghai Fiblaser Technology Co., Ltd., China) was used to deliver a 5-min pulse of blue (473 nm, 10 Hz, 5–8 mW) or yellow light (594 nm, 5–8 mW, constant). In optogenetic inhibition experiments, yellow light was given immediately after establishing the GD model and gastric motility was recorded for 20 min; The control group was not given yellow light after establishing the GD model. In the optogenetic activation experiment, gastric motility was recorded for 20 min immediately after the application of blue light in the light group and in the absence of light in the control group; in the EA group, electroacupuncture was performed for 20 min immediately after the blue light and gastric motility was recorded at the end of the electroacupuncture treatment.
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