Quantitative Gene Expression Analysis

According to phenotypic measurement, 10 samples with the highest and the lowest values of each trait were selected for quantitative analysis. Primer pairs were designed for six genes, AP2/ERF, TLP, PUP9, SLP, HSP, and OCT1. The cDNA was amplified according to the Takara rr820a kit (Takara, Shiga, Japan), and the expression levels of the genes were calculated using the 2^-ΔΔCt method [39 (link)]. The UBI [40 (link)] gene was used to normalize the transcript profiles.

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Ding X., Diao S., Luan Q., Wu H.X., Zhang Y, & Jiang J. (2022). A transcriptome-based association study of growth, wood quality, and oleoresin traits in a slash pine breeding population. PLoS Genetics, 18(2), e1010017.

Publication 2022

rr820a kit

Cdna Expression genes Gene Oct1 Phenotypic Primer

Corresponding Organization : Umeå Plant Science Centre

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Variable analysis

independent variables

Primer pairs designed for six genes: AP2/ERF, TLP, PUP9, SLP, HSP, and OCT1

dependent variables

Expression levels of the genes calculated using the 2^-ΔΔCt method

control variables

UBI gene used to normalize the transcript profiles

controls

No positive or negative controls were explicitly mentioned in the input.

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