Referring to the study of our research group [21 (link)], BSHX extracts were analyzed using a Shimadzu prominence liquid chromatography platform (Kyoto, Japan) equipped with two LC-20AT pumps, a CTO-20A column oven, a DGU-20A5R degasser, an SIL-20A autosampler, and an SPD20AD detector. Chromatographic separation was conducted on a AichromBond-AQ C18 column (250 mm × 4.6 mm, 5 μm; Abel Industries Ltd., Canada), protected by a Phenomenex® C18 guard cartridge (3 × 4 mm, 5 μm; Torrance, CA, USA). The mobile phase consisted of ACN (A) and 0.1% aqueous formic acid (B) and was delivered at 1.0 mL/min with the following gradient program: 0–35 min, 0–3% B; 35–100 min, 3–22% B; 100–115 min, 22–35% B; 115–130 min, 35–35% B; 130–140 min, 35–100% B; 140–145 min, 40–100% B; and 145–155 min, 100–100% B. The column was maintained at 40°C. At the end of each run, the delivery of 100% A was performed for another 14 min for system reequilibration. The monitor wavelength was set at 235 nm, 254 nm, and 280 nm, respectively.
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