For clarity, the numbering convention for amino acids in Ca(v)1.2 used throughout this investigation refers to the rabbit splice variant CACH2A. A plasmid expressing the human HHT-1 splice variant of α1C was kindly provided by Professor Chris Peers, University of Leeds, UK. Regions of this cDNA that encode intracellular loops of the protein were subcloned into the vector pEYFP-C1 (Clontech).
Plasmids to express split α1C (CFP-[I-II]-N-intein and C-intein-[III-IV]-YFP based on the rabbit splice variant CACH2A) and β2a-CFP were kindly provided by Professor Stanley Colecraft, Columbia University. Adenoviruses that express these proteins were generated using the Clontech Adeno-X system and purified and titered using standard techniques. Point mutations were generated using the QuikChange II Site-Directed Mutagenesis Kit (Agilent).
Plasmids to express split α1C (CFP-[I-II]-N-intein and C-intein-[III-IV]-YFP based on the rabbit splice variant CACH2A) and β2a-CFP were kindly provided by Professor Stanley Colecraft, Columbia University. Adenoviruses that express these proteins were generated using the Clontech Adeno-X system and purified and titered using standard techniques. Point mutations were generated using the QuikChange II Site-Directed Mutagenesis Kit (Agilent).
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