Immunohistochemical staining was performed on a Benchmark immunohistochemistry staining system (Bond; Leica, Wetzlar, Germany) with BOND polymer refine detection solution for DAB, using anti‐MGMT (1 : 800, abcam, Cambridge, UK, ab39253) primary antibody as substrate as previously described [45 (link)]. Images were acquired using an Olympus BX46 microscope (Shinjuku City, Tokyo, Japan) as previously described. MGMT immunoreactivity was scored semi‐quantitatively by multiplying the proportion of MGMT positive cells (%) and the staining intensity (0 = none; 1 = weak; 2 = intermediate; and 3 = strong). Statistical comparison was performed using paired Wilcoxon test.
Gallon J., Coto‐Llerena M., Ercan C., Bianco G., Paradiso V., Nuciforo S., Taha‐Melitz S., Meier M., Boldanova T., Pérez‐del‐Pulgar S., Rodríguez‐Tajes S., von Flüe M., Soysal S.D., Kollmar O., Llovet J.M., Villanueva A., Terracciano L.M., Heim M.H., Ng C.K, & Piscuoglio S. (2021). Epigenetic priming in chronic liver disease impacts the transcriptional and genetic landscapes of hepatocellular carcinoma. Molecular Oncology, 16(3), 665-682.
Corresponding Organization : SIB Swiss Institute of Bioinformatics
Other organizations :
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Hospital Clínic de Barcelona, Universitat de Barcelona, Icahn School of Medicine at Mount Sinai, Humanitas University, Istituti di Ricovero e Cura a Carattere Scientifico
MGMT immunoreactivity scored semi-quantitatively by multiplying the proportion of MGMT positive cells (%) and the staining intensity (0 = none; 1 = weak; 2 = intermediate; and 3 = strong)
control variables
Benchmark immunohistochemistry staining system (Bond; Leica, Wetzlar, Germany) with BOND polymer refine detection solution for DAB
Olympus BX46 microscope (Shinjuku City, Tokyo, Japan)
positive controls
Not specified
negative controls
Not specified
Annotations
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