Preparation of 3% w/w Kolliphor EL/ 2% w/w Pluronic P105 surfactant solution is reported previously (Patel S. K. et al. 2013 ). Emulsification protocol developed previously (Janjic et al. 2008 (link); O’Hanlon et al. 2012 (link)) was partially modified. First, surfactant solution was added to the oil(s). If present, solubilizer or PFPE-tyramide dissolved in transcutol (at 50 mg/mL) was added to the mixture. Then, coarse emulsions were produced with an analog vortex mixer (VWR, Radnor, PA) on high for 30 seconds. Triphasic emulsions were sonicated for 30 seconds at 29% amplitude with Model 500 Ultrasonic Dismembrator (FisherScientific, Pittsburgh, PA). Microfluidization was performed on all coarse emulsions by Microfluidizer M110S (Microfluidics Corp., Westwood, MA) at 15,000 psi liquid pressure for 20 pulses over ice cold interaction chamber. Emulsions were stored at 4 °C in glass vials. This method was used to produce all presented formulations. Microfluidization processing parameters were kept constant throughout the study.