Paclitaxel-Resistant MCF-7 Cell Line

The human BC cell line MCF-7 was obtained from COBIOER (Nanjing, China) and incubated in a paclitaxel for over 6 months, and we gradually increased the paclitaxel concentration[4 (link)]. Thereafter, MCF-7/PR cell line was established. The DMEM medium (Gibco, Waltham, CA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco) containing 100 mg/mL streptomycin (Sigma-Aldrich, St-Louis, MO, USA) and 100 U/mL penicillin (Sigma-Aldrich) was used to incubate cells. Cell incubation was under a humid condition containing 5% CO₂ at 37°C.

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Chen Y., Li X., Xu J., Xiao H., Tang C., Liang W., Zhu X., Fang Y., Wang H, & Shi J. (2022). Knockdown of nuclear receptor binding SET domain-containing protein 1 (NSD1) inhibits proliferation and facilitates apoptosis in paclitaxel-resistant breast cancer cells via inactivating the Wnt/β-catenin signaling pathway. Bioengineered, 13(2), 3526-3536.

Publication 2022

MCF-7 DMEM medium FBS streptomycin penicillin

Cell Cell line Human Mcf 7 cell Paclitaxel Penicillin Streptomycin

Corresponding Organization : Nanjing Medical University

Other organizations : Jiangsu Province Hospital

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Variable analysis

independent variables

Paclitaxel concentration

dependent variables

Establishment of MCF-7/PR cell line

control variables

DMEM medium supplemented with 10% fetal bovine serum (FBS), 100 mg/mL streptomycin, and 100 U/mL penicillin
Incubation under humid condition with 5% CO2 at 37°C

controls

No positive or negative controls were explicitly mentioned.

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