The PEG-b-AGE coating polymer was synthesized following the procedure reported by us previously.34 (link) Water-insoluble IONP crystals made from thermodecomposition and stabilized by oleic acids in chloroform were dispersed in tetrahydrofuran via solvent exchange, and transferred to aqueous media after being coated with PEG-b-AGE polymers via the ligand exchange reaction to replace oleic acids on the surface of IONPs as described earlier.34 (link) The core sizes of IONPs before and after coating were measured using a transmission electron microscope (TEM, H-7500, Hitachi, Dallas, TX, USA). The hydrodynamic sizes and surface charges of PEG-b-AGE-coated IONPs were measured using a dynamic light scattering instrument (Zetasizer Nano S90, Malvern, Westborough, MA, USA). Averaged hydrodynamic sizes and zeta potentials were calculated from three measurements for each sample using number-weighted statistics. Iron concentrations were determined by colorimetric spectroscopy as described in the literature.36
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Synthesis and Characterization of PEG-b-AGE-Coated IONPs
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Chloroform
Colorimetric
Hydrodynamic
Ionps
Iron
Ligand
Oleic acids
Polymers
Solvent
Spectroscopy
Tetrahydrofuran
Transmission electron microscope
Corresponding Organization : Emory University
Other organizations : ShenZhen People’s Hospital
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- Synthesis of PEG-b-AGE coating polymer
- Preparation of water-insoluble IONP crystals from thermodecomposition and stabilized by oleic acids in chloroform
- Dispersion of IONP crystals in tetrahydrofuran via solvent exchange
- Coating of IONP crystals with PEG-b-AGE polymers via ligand exchange reaction
- Core sizes of IONPs before and after coating, measured using transmission electron microscope (TEM)
- Hydrodynamic sizes and surface charges of PEG-b-AGE-coated IONPs, measured using dynamic light scattering instrument
- Iron concentrations, determined by colorimetric spectroscopy
- Procedure for synthesis of PEG-b-AGE coating polymer, as reported in a previous study (reference 34)
- Procedure for preparation of water-insoluble IONP crystals and their stabilization by oleic acids in chloroform, as reported in a previous study (reference 34)
- Procedure for coating IONP crystals with PEG-b-AGE polymers via ligand exchange reaction, as reported in a previous study (reference 34)
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