Conidiation structures were examined, measured and photographed on a
compound microscope from cultures grown on CMD, SNA, or PDA or MEA in certain
cases, on the plates under low magnification and after mounting in 3 % KOH.
The following characters were measured: length of conidia, width of conidia,
length of phialides, width of phialides at the base, width of phialides at the
widest point. The size of chlamydospores was measured by examining colonies
grown on CMD or SNA under the conditions described above using the 40×
objective of a compound microscope.
Dry stromata of Hypocrea were rehydrated overnight with water
vapour in a closed glass chamber at room temperature, treated briefly with 3 %
KOH to observe colour changes, embedded in Tissue-Tek O.C.T. Compound 4583
(Sakura Finetek Europe B.V., Zoeterwoude, The Netherlands) and sectioned at a
thickness of 10–12 μm with a freezing microtome. Sections were
measured and photographed in lactic acid, or 3 % KOH where noted.
Whenever possible, gross morphology including colour and approximate sizes
of fresh stromata were determined in the field in order to estimate changes
caused by drying. In dry stromata the diameter, height, colour and shape of
the stromata were determined. In sections the following teleomorph
characteristics were evaluated: perithecium shape, length and width; colour,
width of perithecium wall; length and diameter of ostioles; thickness and
structure of the surface region (cortex), structure and size of cells of the
subcortical (between the perithecia and the cortex), subperithecial (below the
perithecia) and basal regions of the stroma. Length and width of asci and
distal and proximal ascospore cells were measured in separate preparations in
3 % KOH (or water or lactic acid where noted). Measurements are reported as
maxima and minima in parentheses and the mean plus and minus the standard
deviation of a number of measurements given in parentheses. Nomarski
differential interference contrast (DIC) was used for observations and
measurements. Images were recorded with the Nikon Coolpix 4500 or DS-U2
digital cameras. Measurements were carried out directly through microscope
oculars or using the NIS-Elements D (v. 2.2 or 3.0) software. Colours were
determined with Methuen's Handbook of Colour
(Kornerup & Wanscher
1981 ). Colour terms are not strictly followed, therefore also the
codes are cited.
compound microscope from cultures grown on CMD, SNA, or PDA or MEA in certain
cases, on the plates under low magnification and after mounting in 3 % KOH.
The following characters were measured: length of conidia, width of conidia,
length of phialides, width of phialides at the base, width of phialides at the
widest point. The size of chlamydospores was measured by examining colonies
grown on CMD or SNA under the conditions described above using the 40×
objective of a compound microscope.
Dry stromata of Hypocrea were rehydrated overnight with water
vapour in a closed glass chamber at room temperature, treated briefly with 3 %
KOH to observe colour changes, embedded in Tissue-Tek O.C.T. Compound 4583
(Sakura Finetek Europe B.V., Zoeterwoude, The Netherlands) and sectioned at a
thickness of 10–12 μm with a freezing microtome. Sections were
measured and photographed in lactic acid, or 3 % KOH where noted.
Whenever possible, gross morphology including colour and approximate sizes
of fresh stromata were determined in the field in order to estimate changes
caused by drying. In dry stromata the diameter, height, colour and shape of
the stromata were determined. In sections the following teleomorph
characteristics were evaluated: perithecium shape, length and width; colour,
width of perithecium wall; length and diameter of ostioles; thickness and
structure of the surface region (cortex), structure and size of cells of the
subcortical (between the perithecia and the cortex), subperithecial (below the
perithecia) and basal regions of the stroma. Length and width of asci and
distal and proximal ascospore cells were measured in separate preparations in
3 % KOH (or water or lactic acid where noted). Measurements are reported as
maxima and minima in parentheses and the mean plus and minus the standard
deviation of a number of measurements given in parentheses. Nomarski
differential interference contrast (DIC) was used for observations and
measurements. Images were recorded with the Nikon Coolpix 4500 or DS-U2
digital cameras. Measurements were carried out directly through microscope
oculars or using the NIS-Elements D (v. 2.2 or 3.0) software. Colours were
determined with Methuen's Handbook of Colour
(
1981
codes are cited.
