E2F1+/+ and E2F1−/− mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). C57BL/6 (WT), SHP−/− (SKO), SHP non-transgenic control (NC), hepatocyte specific SHP transgenic (STG), FXR−/− mice were described previously (14 (link), 21 (link), 22 (link)). The treatment of mice with DDC supplemented diet, bile duct ligation (BDL), and FXR synthetic ligand GW4064 has been described previously (19 (link), 20 (link), 22 (link)). Protocols for animal use were approved by the Institutional Animal Care and Use Committee at the University of Kansas Medical Center and University of Utah.
Protocol detail
Genetic Mouse Models for Liver Research
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Animal
Bile duct
Diet
E2f1
Gw4064
Hepatocyte
Institutional animal care and use committee
Ligand
Ligation
Mice
Transgenic
Transgenic mice
Corresponding Organization : University of Utah
Other organizations : Zhejiang University, Henan Agricultural University, Rutgers, The State University of New Jersey, Michigan State University
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Protocol cited in 6 other protocols
Variable analysis
independent variables
- E2F1 genotype (+/+ vs -/-)
- SHP genotype (-/- vs non-transgenic control vs hepatocyte specific transgenic)
- FXR genotype (-/-)
- Diet (DDC supplemented)
- Surgery (bile duct ligation, BDL)
- FXR synthetic ligand (GW4064)
- Not explicitly mentioned
- C57BL/6 (WT) mice
- SHP non-transgenic control (NC) mice
- SHP non-transgenic control (NC) mice
- C57BL/6 (WT) mice
- SHP knockout (-/-) mice
- FXR knockout (-/-) mice
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