On the day of the experiment, flies of different sex, age, and genotype were allocated to vials ensuring equal numbers for the different treatment groups. Flies were exposed to volatile general anesthetics and oxygen at specific concentrations using a device that permits simultaneous exposure of up to eight groups of flies.14 (link) Volatile general anesthetics were administered using a Datex-Ohmeda Aestiva/5 anesthesia machine equipped with commercial agent-specific vaporizers (Datex-Ohmeda Inc., Madison, WI). Compressed gas cylinders (Airgas USA, LLC, Radnor, PA) containing 100% O2, 100% N2, or air (21%O2/79%N2) provided carrier gas of the desired composition. Anesthetic exposure consisted of either 2% isoflurane or 3.5% sevoflurane for 2 h at room temperature. Isoflurane and sevoflurane were obtained from Piramal Enterprises Ltd. (Maharashtra, India). To control for effects of circadian rhythm, flies were anesthetized at a similar time of day (between 10:00 AM and 2:00 PM).
Protocol detail
Volatile Anesthesia Exposure in Flies
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Anesthesia
Anesthetic
Circadian rhythm
Device
Flies
General anesthetics
Genotype
Isoflurane
Sevoflurane
Sex different
Vaporizers
Corresponding Organization :
Other organizations : American Society of Anesthesiologists, University of Wisconsin–Madison, Committee on Publication Ethics
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Variable analysis
independent variables
- Volatile general anesthetics (2% isoflurane or 3.5% sevoflurane)
- Not explicitly mentioned
- Sex of flies
- Age of flies
- Genotype of flies
- Exposure duration (2 hours)
- Time of day (between 10:00 AM and 2:00 PM)
- Temperature (room temperature)
- Positive control: Flies exposed to 100% O2 or air (21% O2/79% N2)
- Negative control: Not explicitly mentioned
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