Protein Expression Analysis by Western Blot and Immunofluorescence

Western blotting and immunofluorescence analyses were performed as published23 (link) with the following antibodies: anti-OCT4 (Abcam, Cambridge, UK), anti-Tra-1-60 (Merck Millipore, Darmstadt, Germany), anti-NANOG (Stemgent, Lexington, USA), anti-SSEA-4 (Merck Millipore, Darmstadt, Germany), anti-TUJ1 (Covance Inc., Princeton, USA), anti-Tbr1 (Abcam, Cambridge, UK), anti-Brn2 (Santa Cruz), anti-ε-sarcoglycan (esg2-1355, published antibody against the brain-specific isoform of the protein)24 (link), anti-FLAG (Sigma Aldrich, St. Louis, USA), anti-Flotilin-1 (Cell Signaling Technology, Danvers, USA), and anti-β-actin (Sigma Aldrich, St. Louis, USA).