Isolation and Characterization of Quercetin Glycosides

The reference samples of quercetin-3-O-[β-D-glu-(1 → 6)]-β-D-glucopyranoside (AFG-1), quercetin-3-O-[β-D-xyl-(1 → 2)]-β-D-glucopyranoside (AFG-2), and quercetin-4^″-O-methy-3-O-β-D-glucopyranoside (AFG-3) at a purity of >98% were separated by our research team [16 (link)]. Rutin reference sample was purchased from China Pharmaceutical and Biological Products Testing Institute. A. esculentus flower, fruit, and seed samples were picked up in vegetable test base of Zhejiang Agricultural and Forestry University in 2016. Nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), and Coomassie Brilliant Blue kit were purchased from Nanjing Jiancheng Biological Technology Co. Ltd. Antibodies against Nrf2, heme oxygenase-1 (HO-1), NAD(P)H:quinone oxidoreductase-1 (NQO1), and β-actin were purchased from Wanlei Biological Technology Co. Ltd. Chloral hydrate was purchased from Zhejiang Academy of Medical Sciences. All the reagents were of analytical or HPLC grade.

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Luo Y., Cui H.X., Jia A., Jia S.S, & Yuan K. (2018). The Protective Effect of the Total Flavonoids of Abelmoschus esculentus L. Flowers on Transient Cerebral Ischemia-Reperfusion Injury Is due to Activation of the Nrf2-ARE Pathway. Oxidative Medicine and Cellular Longevity, 2018, 8987173.

Publication 2018

Coomassie Brilliant Blue kit β-actin

Actin Antibodies Biological Biological products Chloral hydrate Coomassie brilliant blue Fruit Heme oxygenase 1 Hplc Malondialdehyde Nad p h oxidoreductase Nitric oxide Nqo1 Nrf2 Pharmaceutical Quercetin Quinone Rutin Superoxide dismutase Vegetable

Corresponding Organization : Jiyang College of Zhejiang A&F University

Other organizations : First Affiliated Hospital of Henan University, Huanghe Science and Technology College

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Variable analysis

independent variables

A. esculentus flower samples
A. esculentus fruit samples
A. esculentus seed samples

dependent variables

Nitric oxide (NO) levels
Malondialdehyde (MDA) levels
Superoxide dismutase (SOD) activity
Nrf2 expression
Heme oxygenase-1 (HO-1) expression
NAD(P)H:quinone oxidoreductase-1 (NQO1) expression

control variables

Purity of reference samples (>98%)
Rutin reference sample
Chloral hydrate
All reagents were of analytical or HPLC grade

positive control

Rutin reference sample

negative control

Not explicitly mentioned

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