The immortalized motoneuronal cell line NSC34 (58 (link),59 (link)) is routinely used in our laboratory (24 (link),37 (link),39 (link)–41 (link),52 (link),54 (link),60 (link)–62 (link)) and has been transfected with Lipofectamine (Life Technologies Corporation, Carlsbad, CA, USA)/transferrin (Sigma-Aldrich), as previously described (39 (link),40 (link),60 (link)), using 0.6 µg of plasmid DNA, 4 µl of transferrin solution and 2 µl of Lipofectamine.
Rat adrenal pheochromocytoma (PC12) cells stable transfected with the plasmid encoding AR.Qn (n010, 112) express AR under the control of a Tet-On promoter responsive to 1 µg/ml of doxycycline [produced (and kindly provided) by Professor D. Merry (TJU, Philadelphia, PA, USA)]. In the experiments involving steroid hormone treatments, the fetal bovine serum (FBS) was replaced with charcoal-stripped FBS and the horse serum (HS) with charcoal-stripped HS, to eliminate endogenous steroids (24 (link),40 (link),63 (link)).
Rat adrenal pheochromocytoma (PC12) cells stable transfected with the plasmid encoding AR.Qn (n010, 112) express AR under the control of a Tet-On promoter responsive to 1 µg/ml of doxycycline [produced (and kindly provided) by Professor D. Merry (TJU, Philadelphia, PA, USA)]. In the experiments involving steroid hormone treatments, the fetal bovine serum (FBS) was replaced with charcoal-stripped FBS and the horse serum (HS) with charcoal-stripped HS, to eliminate endogenous steroids (24 (link),40 (link),63 (link)).
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